Photo-degradation of water and food pathogens using cheap hand held laser

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Physics Department

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Physics Department

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Physics Department

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https://doi.org/10.1117/12.3043613

All Authors

Sara Mohamed C. Tharwat Amany Khalifa Yasmine Elbagoury Shaimaa F. Ahmed Mohamed A. Swillam

Document Type

Research Article

Publication Title

Proceedings of SPIE the International Society for Optical Engineering

Publication Date

1-1-2025

doi

10.1117/12.3043613

Abstract

Applications in the fields of medicine, food and agriculture, water and hygiene, and light-based microbe inhibition have drawn more attention than those that rely on chemical or heat inhibition. The photo-biological treatment of dangerous microbes using blue light (400–500 nm) for many purposes, such as food safety regulation, has shown encouraging results time and time again. Certain bacteria that do not require photosynthesis possess domains of the blue light receptor protein. The way that various bacteria react physiologically to blue light depends on their dietary surroundings. The presence of water is one of the biggest risk factors for microbiological contamination in dry operations; as a result, the use of traditional wet cleaning and sanitation techniques is limited. Blue light irradiation (BLI) is an FDA-approved treatment for some illnesses, such as acne, and is gaining popularity as an antibacterial tactic due to the growth of antibiotic-resistant "superbugs." The usefulness of BLI in various bacterial development stages, particularly in more BLI-tolerant species like E. coli, has not, however, been well studied. On the other hand, the shadow effect can prevent light from being absorbed, which might impede light irradiation inactivation. Because food components are very opaque, this kind of inactivation works best against bacteria found on their surfaces and in clear liquid meals like fruit juices without particles. When food is processed and stored, bacteria on its surfaces can get inside its intercellular gaps and fissures. There is still a dearth of research on how well blue light works to lower microbial contamination in dry food processing facilities. Consequently, this study's goals will be: elevate the effectiveness of blue high-power laser source for both E. coli & Salmonella photo-degradation. Taking into consideration these critical factors: (i)exposure time, different concentration of bacteria, and (ii) the distance between the laser source and the bacterial strains.

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