Detection of Escherichia coli Bacteria using Surface Plasmon Resonance-Wavelength Interrogation setup

Author's Department

Physics Department

Fifth Author's Department

Physics Department

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https://doi.org/10.1117/12.3044165

All Authors

Sara Mohamed Karim El-Seherawy Mostafa Hassan Ahmed Kreta Baraah Hasanin Yasmine Elbagoury Heba Refaat Shaimaa F. Ahmed Mai Mostafa Ahmed Moustafa Mohamed A. Swillam

Document Type

Research Article

Publication Title

Proceedings of SPIE the International Society for Optical Engineering

Publication Date

1-1-2025

doi

10.1117/12.3044165

Abstract

The last 20 years have seen significant progress in Surface Plasmonic Resonance (SPR), which has applications in material characterization, chemical sensing, biosensing, and other domains. SPR's increased sensitivity to changes in the materials' refractive indices has made it common in biosensing applications. In this study, we built an affordable SPR sensor system in our lab using wavelength interrogation setup. The suggested setup was developed and included a spectrophotometer, polarization sheet, attenuator, and a supercontinuum white light source. The SPR phenomenon is attained when a p-polarized light beam incident above a semicircular prism, which serves as a coupling medium and is attached to a thin layer of 53 nm thickness of gold (Au) by a matching oil. The SPR phenomenon is based on the wavelength, refractive index, and the angle as well. The dielectric medium can be changed, which changes the SPR angle as a result. Fixing the SPR angle while detecting the SPR wavelength over a broad wavelength range (400-700 nm) is our main objective. Pathogenic bacteria such as Escherichia coli (E. coli) can have fatal effects on human health. It is possible that the water that is contaminated contains E. coli. In order to mimic the contaminated water, E. coli analytes with various concentrations were prepared using the serial dilution method and were diluted in sterilized deionized water with concentrations of (10-1-10-10) cells/mL. Achieving the lowest concentration of E. coli analytes to ascertain the limit of detection (LOD) and the sensitivity of the created SPR setup are the goals of the serial dilution approach.

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