Nuclear magnetic resonance metabolomics approach for the analysis of major legume sprouts coupled to chemometrics
Funding Number
RSG1–18
Funding Sponsor
Alexander von Humboldt-Stiftung
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https://doi.org/10.3390/molecules26030761
Document Type
Research Article
Publication Title
Molecules
Publication Date
2-1-2021
doi
10.3390/molecules26030761
Abstract
Legume sprouts are a fresh nutritive source of phytochemicals of increasing attention worldwide owing to their many health benefits. Nuclear magnetic resonance (NMR) was utilized for the metabolite fingerprinting of 4 major legume sprouts, belonging to family Fabaceae, to be exploited for quality control purposes. Thirty-two metabolites were identified belonging to different classes, i.e., fatty acids, sugars, amino acids, nucleobases, organic acids, sterols, alkaloids, and isoflavonoids. Quantitative NMR was employed for assessing the major identified metabolite levels and multivariate data analysis was utilized to assess metabolome heterogeneity among sprout samples. Isoflavones were detected exclusively in Cicer sprouts, whereas Trigonella was characterized by 4-hydroxyisoleucine. Vicia sprouts were distinguished from other legume sprouts by the presence of L-Dopa versus acetate abundance in Lens. A common alkaloid in all sprouts was trigonelline, detected at 8–25 µg/mg, suggesting its potential role in legume seeds’ germination. Trigonelline was found at highest levels in Trigonella sprouts. The aromatic NMR region data (δ 11.0–5.0 ppm) provided a better classification power than the full range (δ 11.0–0.0 ppm) as sprout variations mostly originated from secondary metabolites, which can serve as chemotaxonomic markers.
Recommended Citation
APA Citation
Farag, M.
Sharaf El-Din, M.
Selim, M.
Owis, A.
...
(2021). Nuclear magnetic resonance metabolomics approach for the analysis of major legume sprouts coupled to chemometrics. Molecules, 26(3),
10.3390/molecules26030761
https://fount.aucegypt.edu/faculty_journal_articles/2584
MLA Citation
Farag, Mohamed A., et al.
"Nuclear magnetic resonance metabolomics approach for the analysis of major legume sprouts coupled to chemometrics." Molecules, vol. 26,no. 3, 2021,
https://fount.aucegypt.edu/faculty_journal_articles/2584