Distinct domains of Escherichia coli IgaA connect envelope stress sensing and down-regulation of the Rcs phosphorelay across subcellular compartments.
Author's Department
Biology Department
Second Author's Department
Biology Department
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https://journals.plos.org/plosgenetics/article/file?id=10.1371/journal.pgen.1007398&type=printable
Document Type
Research Article
Publication Title
PLOS Genetics.
Publication Date
5-31-2018
doi
10.1371/journal.pgen.1007398
Abstract
In enterobacteria, the Rcs system (Regulator of capsule synthesis) monitors envelope integrity and induces a stress response when damages occur in the outer membrane or in the peptidoglycan layer. Built around a two-component system, Rcs controls gene expression via a cascade of phosphoryl transfer reactions. Being particularly complex, Rcs also involves the outer membrane lipoprotein RcsF and the inner membrane essential protein IgaA (Intracellular growth attenuator). RcsF and IgaA, which are located upstream of the phosphorelay, are required for normal Rcs functioning. Here, we establish the stress-dependent formation of a complex between RcsF and the periplasmic domain of IgaA as the molecular signal triggering Rcs. Moreover, molecular dissection of IgaA reveals that its negative regulatory role on Rcs is mostly carried by its first N-terminal cytoplasmic domain. Altogether, our results support a model in which IgaA regulates Rcs activation by playing a direct role in the transfer of signals from the cell envelope to the cytoplasm. This remarkable feature further distinguishes Rcs from other envelope stress response systems.
First Page
1
Last Page
17
Recommended Citation
APA Citation
Hussein, N. A.
&
Siam, R. M.
(2018). Distinct domains of Escherichia coli IgaA connect envelope stress sensing and down-regulation of the Rcs phosphorelay across subcellular compartments.. PLOS Genetics., 14(5), 1–17.
10.1371/journal.pgen.1007398
https://fount.aucegypt.edu/faculty_journal_articles/172
MLA Citation
Hussein, Nahla, et al.
"Distinct domains of Escherichia coli IgaA connect envelope stress sensing and down-regulation of the Rcs phosphorelay across subcellular compartments.." PLOS Genetics., vol. 14,no. 5, 2018, pp. 1–17.
https://fount.aucegypt.edu/faculty_journal_articles/172