Development of chitosan 2D film scaffolds and nanoparticles enriched with royal jelly and grape seed extract: Enhanced antibacterial and wound healing activity

Abstract

Wound healing using nanomaterials have been increasingly studied due to their remarkable applicability in tissue engineering. Biopolymeric scaffolds have been widely used to fabricate wound dressings and skin substitute. Chitosan the deacetylated form of chitin has received great attention especially in wound healing. Natural extracts such as garlic, curcumin, honey, royal jelly and grape seed extract were used to enhance the fabricated wound dressing materials. In this work, Vitis vinifera (Grape seed extract) and royal jelly were loaded within fabricated low and high molecular weight chitosan 2D film scaffolds and nanoparticles. Vitis vinifera was known with its dermal wound healing properties as well as its antioxidant activity. Royal jelly has been demonstrated to possess in vitro and in vivo antimicrobial, anti-inflammatory and wound healing capabilities in experimental animals. Scaffolds were physicochemically characterized using Fourier transform infrared spectroscopy (FTIR), and X ray diffraction (XRD). Scaffolds morphology was visualized using scanning electron microscope (SEM). Particle size distribution and zeta potential for the chitosan nanoparticles were predicted. In vitro antibacterial testing of the prepared scaffolds and nanoparticles was performed against Bacillus subtilis, Staphylococcus aureus, Enterobacter aerogenes, and Pseudomonas aeruginosa. In addition, the prepared scaffolds and nanoparticles showed no cytotoxicity for human lung fibroblast (Wi38). A preliminary in vivo study revealed that the developed scaffolds and nanoparticles enhanced the wound healing process as compared to the untreated control rat wound closure rate.

Department

Nanotechnology Program

Degree Name

MS in Nanotechnology

Graduation Date

2-1-2019

Submission Date

September 2018

First Advisor

Abdellatif, Ahmed

Committee Member 1

Madkour, Tarek

Committee Member 2

Abd Elsamie, Emtithal

Extent

78 p.

Document Type

Master's Thesis

Rights

The author retains all rights with regard to copyright. The author certifies that written permission from the owner(s) of third-party copyrighted matter included in the thesis, dissertation, paper, or record of study has been obtained. The author further certifies that IRB approval has been obtained for this thesis, or that IRB approval is not necessary for this thesis. Insofar as this thesis, dissertation, paper, or record of study is an educational record as defined in the Family Educational Rights and Privacy Act (FERPA) (20 USC 1232g), the author has granted consent to disclosure of it to anyone who requests a copy.

Institutional Review Board (IRB) Approval

Not necessary for this item

Comments

This work was funded by AUC graduate student research grant.

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