Abstract
One of the hallmarks of cancer cells is their altered metabolism, which serves their need to grow and proliferate. Cancer cells exhibit a marked increase in glucose consumption (Warburg effect). In addition, cancer cells also exhibit excessive uptake of glutamine, for biomass synthesis and for energy production through mitochondrial substrate level phosphorylation (glutaminolysis pathway). Glutamine metabolism is enhanced upon inhibition of glycolysis, also interference of glutamic metabolism leads to upregulation of glucose metabolism. So, simultaneous inhibition of glycolysis and glutaminolysis presents promising metabolic intervention strategy. In this study, we investigated high dose Vitamin C mechanism of action through MTT assays revealing Vitamin C as a selective inhibitor of glycolysis in cancer cells through NAD+ depletion. Also, we tested cytotoxicity of inhibitors of glutaminolysis and in combination with high dose vitamin C in two different cell lines, showing more effectiveness in combination than each treatment alone.
In conclusion, we are proposing a novel combination of high dose ascorbic acid and inhibitor of glutaminolysis as a therapeutic cancer intervention.
School
School of Sciences and Engineering
Department
Biotechnology Program
Degree Name
MS in Biotechnology
Graduation Date
Spring 5-16-2023
Submission Date
5-23-2023
First Advisor
asma amleh
Second Advisor
khaled abou-aishah
Committee Member 1
ahmed abdellatif
Committee Member 2
wael mohamed elsayed
Extent
49 p
Document Type
Master's Thesis
Institutional Review Board (IRB) Approval
Not necessary for this item
Recommended Citation
APA Citation
Mohamed, M. A.
(2023).Targeting the Warburg and Q Effect: Novel Strategy to Disrupt Cancer Metabolism [Master's Thesis, the American University in Cairo]. AUC Knowledge Fountain.
https://fount.aucegypt.edu/etds/2135
MLA Citation
Mohamed, Mahmoud Adel. Targeting the Warburg and Q Effect: Novel Strategy to Disrupt Cancer Metabolism. 2023. American University in Cairo, Master's Thesis. AUC Knowledge Fountain.
https://fount.aucegypt.edu/etds/2135