Abstract

Despite the huge advancement in the field of cancer treatment, the conventionally used treatments still present significant challenges such as low effectiveness and adverse side effects. Thus, there is a critical need to continuously develop new, more effective and safe drugs against cancer. Herbal plants serve as a potential source for a wide variety of complex compounds with probable anticancer activity. The genus Salvia encompasses about 900 species. Salvia triloba, commonly known as Greek sage, is native to the Middle East and is inherently linked to local traditional medicine systems. It was recently shown to possess anti-proliferative activity against breast cancer cell lines. However, there is no experimental evidence regarding the anticancer effects of Salvia triloba on osteosarcoma. In this study, we investigated the effect of Salvia triloba ethanol and acetone extracts, as well as the thin-layer chromatography-fractionated acetone extract on viability, and the effect of both crude extracts on the migratory ability of U2OS osteosarcoma cells. MTT results have shown that the acetone crude extract exhibits the most potent cytotoxic effect, indicating the positive cooperative interactions between the different compounds present in the plant material. Moreover, trans-well migration assay illustrated that acetone crude extract was more powerful than the ethanol extract in hindering cellular migration. Furthermore, we show that Salvia triloba has no significant hemolytic activity and most likely will target anionic cancer cells while sparing the healthy neutral cells (erythrocytes). In conclusion, our results have shown that Salvia triloba inhibits U2OS cells’ proliferation, migration and showed no considerable hemolytic activity on human erythrocytes upon application. Altogether, these results promote the novel therapeutic potential of Salvia triloba as an anticancer drug.

School

School of Sciences and Engineering

Department

Biotechnology Program

Degree Name

MS in Biotechnology

Graduation Date

Spring 5-31-2020

Submission Date

5-31-2020

First Advisor

Amleh, Asma

Committee Member 1

El Sayed, Mayyada

Committee Member 2

Mahmoud, Hamada

Extent

69 p.

Document Type

Master's Thesis

Rights

The American University in Cairo grants authors of theses and dissertations a maximum embargo period of two years from the date of submission, upon request. After the embargo elapses, these documents are made available publicly. If you are the author of this thesis or dissertation, and would like to request an exceptional extension of the embargo period, please write to thesisadmin@aucegypt.edu

Institutional Review Board (IRB) Approval

Approval has been obtained for this item

Comments

I would like to express my sincere appreciation and gratitude to my professor, advisor, and mentor Dr. Asma Amleh for all her effort and the time she has dedicated and invested in teaching and guiding me throughout the past years. I appreciate a lot being a member of Dr. Amleha' s research team, this chance was a gate to gain a lot of knowledge and grow as a scientist. Thank you, Dr.Asma for all your patience, support, guidance, and time. I would like to thank Dr. Rami Arafa, our collaborator, who provided us with the plant samples and did the fractionation. I want to express my gratitude to all the AUC professors, who taught me a lot throughout my study in the AUC. I would like to thank all my teammates, thank you for the advice and support. I would especially like to thank Eric Zadok, Myret Ghabriel, and Omnia Abd El-Raheem, Eric is a senior member of our team who taught me all the techniques I needed to start my project. Last but not least, I would like to thank the AUC for providing us with research funds and fellowship opportunities.

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