Abstract

Ultra-high molecular weight polyethylene (UHMWPE) semi crystalline biomaterial is one of the gold standard materials that are used as a bearing surface in total joint replacement surgeries. However, wear particles generated by UHMWPE due to the relative motion between the different components of the bearing, would eventually result in osteolysis and implant failure. For this, many attempts to enhance the properties of UHMWPE were done, including coating the UHMWPE with nylon 6,6 which was reported to improve its mechanical properties and biocompatibility. In this study, the antibacterial activity, moisture and SBF absorption, pH effect, bioactivity, biocompatibility and wound healing ability of the nylon coated in contrast to the uncoated UHMWPE were assessed. The results have shown that the coated UHMWPE was more effective (P<0.001) than the uncoated UHMWPE regarding bacterial (Staphylococcus aureus and Escherichia coli) growth inhibition. Moreover, coated UHMWPE demonstrated superiority over the uncoated UHMWPE; by absorbing less moisture of both simulated body fluid and lactated Ringer's solution and by rendering the pH of simulated body fluid (SBF) less acidic. Chemical analysis of the nylon coated UHMWPE by FTIR, and morphological assessments by SEM confirmed the absence of hydroxyapatite layer and hence the inability of the nylon coated UHMWPE to be osteoconductive. The assessment of the U2-OS cell viability using MTT assay has suggested that both materials appear to not cause cell cytotoxicity and may be accelerating the cellular proliferation after 72 hours when compared to the control sample. For wound healing, nylon coated UHMWPE proved its ability to be a better orthopedic suture than the uncoated sample and control by showing a better wound closure percentage. These interdisciplinary approaches have given us the chance to investigate different features of nylon coated UHMWPE, which is a promising tool to enhance bearing surfaces in total joint arthroplasties.

Department

Biotechnology Program

Degree Name

MS in Biotechnology

Graduation Date

Fall 1-13-2019

Submission Date

January 2019

First Advisor

Amleh, Asma

Committee Member 1

Mamdouh, Wael

Committee Member 2

Abdelaziz, Ahmed

Extent

72 p.

Document Type

Master's Thesis

Rights

The author retains all rights with regard to copyright. The author certifies that written permission from the owner(s) of third-party copyrighted matter included in the thesis, dissertation, paper, or record of study has been obtained. The author further certifies that IRB approval has been obtained for this thesis, or that IRB approval is not necessary for this thesis. Insofar as this thesis, dissertation, paper, or record of study is an educational record as defined in the Family Educational Rights and Privacy Act (FERPA) (20 USC 1232g), the author has granted consent to disclosure of it to anyone who requests a copy. The author has granted the American University in Cairo or its agents a non-exclusive license to archive this thesis, dissertation, paper, or record of study, and to make it accessible, in whole or in part, in all forms of media, now or hereafter known.

Institutional Review Board (IRB) Approval

Not necessary for this item

Comments

I would like to acknowledge AUC for laboratory instruction fellowship.

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