Abstract

Fibroblasts are the most common connective tissue cells and are responsible for the synthesis of extracellular matrix (ECM) and collagen. They are mainly of mesenchymal stem cells (MSCs) origin. Fibroblasts are fully differentiated cells although some recent findings reported that fibroblasts possess some potency that allows them to differentiate into other cells types. They are said to undergo adipogenic, chondrogenic, hepatogenic, nurogenic and osteogenic differentiation if grown in the suitable conditions. In the normal physiologic environment, fibroblasts can also differentiate to epithelial cells, a phenomenon known as the mesenchymal epithelial transition (MET). In the current research, we induced L929 mouse fibroblasts to differentiate into osteoblasts cells using the growth media containing Dexamethazone, Ascorbic acid and Beta-Glycerol phosphate which are the substances known to promote osteogenic differentiation. We have increased the concentration of dexamethazone than that used previously, and investigated the effect of this increase on the time of induction. We also investigated the levels of Cobra1 (which is an integral member of the negative elongation (NELF) complex) mRNA in L929 that underwent osteogenic induction compared to the non-induced ones. We show a successful induction the onset of which is earlier than previously reported in the literature. Furthermore, to the best our knowledge our current data, for the first time, suggest a potential role of Cobra1 in maintaining the plasticity of L929.

Department

Biotechnology Program

Degree Name

MS in Biotechnology

Graduation Date

Fall 9-14-2019

Submission Date

June 2010

First Advisor

Amleh, Asma

Second Advisor

NA

Third Advisor

NA

Committee Member 1

NA

Committee Member 2

NA

Committee Member 3

NA

Extent

52 p.

Document Type

Master's Thesis

Rights

The author retains all rights with regard to copyright. The author certifies that written permission from the owner(s) of third-party copyrighted matter included in the thesis, dissertation, paper, or record of study has been obtained. The author further certifies that IRB approval has been obtained for this thesis, or that IRB approval is not necessary for this thesis. Insofar as this thesis, dissertation, paper, or record of study is an educational record as defined in the Family Educational Rights and Privacy Act (FERPA) (20 USC 1232g), the author has granted consent to disclosure of it to anyone who requests a copy. The author has granted the American University in Cairo or its agents a non-exclusive license to archive this thesis, dissertation, paper, or record of study, and to make it accessible, in whole or in part, in all forms of media, now or hereafter known.

Institutional Review Board (IRB) Approval

Not necessary for this item

Comments

I would like to thank Dr. Asma Amleh for her support and patience. I am also grateful for Dr. Adham Ramadan's support. Great help was offered to me from my colleagues; Noha Saad, Nancy Hasanein, Myret Ghabriel, Menna Ghoraba and Menna Alfar. So thank them all so much. Finally, I would like to express much gratitude to the AUC for providing the grant that funded this research.

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