Abstract
The circadian clock plays an essential role in most organisms by allowing their adaptation to the daily environmental changes mainly light/dark cycles and also temperature. Circadian rhythms persist in constant conditions indicating an auto regulatory molecular circadian clock. Here, we investigated the possibility that the circadian clock of Drosophila can also be entrained by scheduled feeding. Cycles of 12 hours feeding and 12 hours starvation could entrain the molecular circadian oscillation in key pacemaker neurons of Drosophila. Clock proteins CLOCK (CLK) and PERIOD (PER) showed a 24 hour oscillation in the small and large ventral lateral neurons after entrainment by scheduled feeding of a balanced diet, carbohydrate-only diet, as well as protein only diet. Lipid diet did not prove its effectiveness in the clock's entrainment. The neuropeptide Pigment Dispersing Factor (PDF) plays an important role in entrainment of the circadian clock by light-dark cycles, but it appears to be not essential for entrainment of the circadian clock in response to cyclic feeding. Scheduled feeding resulted in 24 hour oscillations of CLK and PER in small and large ventral lateral neurons of pigment dispersing factor mutant flies (pdf01). Time of feeding appears to be a strong entrainment cue for the circadian clock as it affected the phase of amplitude of molecular oscillations even in light dark cycles. These results establish an entrainment of the Drosophila circadian clock by scheduled feeding, indicating a regulation of the circadian oscillator by metabolism in Drosophila.
Department
Chemistry Department
Degree Name
MS in Chemistry
Graduation Date
6-1-2014
Submission Date
May 2014
First Advisor
Azzazy, Hassan
Committee Member 1
Azzazy, Hassan
Committee Member 2
Weber, Frank
Extent
163 p.
Document Type
Master's Thesis
Library of Congress Subject Heading 1
Circadian rhythms.
Library of Congress Subject Heading 2
Drosophila melanogaster.
Rights
The author retains all rights with regard to copyright. The author certifies that written permission from the owner(s) of third-party copyrighted matter included in the thesis, dissertation, paper, or record of study has been obtained. The author further certifies that IRB approval has been obtained for this thesis, or that IRB approval is not necessary for this thesis. Insofar as this thesis, dissertation, paper, or record of study is an educational record as defined in the Family Educational Rights and Privacy Act (FERPA) (20 USC 1232g), the author has granted consent to disclosure of it to anyone who requests a copy.
Institutional Review Board (IRB) Approval
Not necessary for this item
Recommended Citation
APA Citation
Diab, A.
(2014).The impact of nutrition and feeding time on the regulation of gene expression in the central circadian clock of Drsosophila Melanogaster [Master's Thesis, the American University in Cairo]. AUC Knowledge Fountain.
https://fount.aucegypt.edu/etds/1195
MLA Citation
Diab, Arwa. The impact of nutrition and feeding time on the regulation of gene expression in the central circadian clock of Drsosophila Melanogaster. 2014. American University in Cairo, Master's Thesis. AUC Knowledge Fountain.
https://fount.aucegypt.edu/etds/1195