Development of an efficient indirect somatic embryogenesis and shoot regeneration system for sweet sorghum cultivars using immature inflorescence
Funding Sponsor
American University in Cairo
Author's Department
Computer Science & Engineering Department
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https://doi.org/10.1007/s11627-024-10496-z
Document Type
Research Article
Publication Title
In Vitro Cellular and Developmental Biology Plant
Publication Date
6-1-2025
doi
10.1007/s11627-024-10496-z
Abstract
Sweet sorghum (Sorghum bicolor L. Moench) is a C4 crop that is characterized by its high photosynthetic efficiency and is specifically bred for its high lignocellulosic biomass in addition to its broad utilization in food, fodder, and biofuel production. Still establishing a robust in vitro tissue culture system for sorghum requires further improvement due to its major limitations in phenolic release and low regeneration rate. In this study, immature inflorescences were employed using three different sweet sorghum varieties (Rex, Ramada, and Sugar Drip) to establish an efficient regeneration system. The study revealed optimal rates of embryogenic callus induction in the varieties Rex (77%), Ramada (93%), and Sugar Drip (94%) from immature inflorescence explants cultured on Murashige and Skoog (MS) medium supplemented with 4.0 or 6.0 mg L−1 2,4-D and 0.2 mg L−1 Kin. The aforementioned concentrations also support the establishment of an embryogenic callus (74%) from a greater inflorescence length (9 to 12 cm) among the three genotypes. The best shoot response, shoot number/explant, and acclimatization process for the three varieties were ascertained following culture on MS medium containing 1.0 mg L−1 IAA, 0.5 mg L−1 BAP, and 0.1 mg L−1 TDZ. Consequently, the rate of shoot induction was 30, 43, and 28% in Rex, Ramada, and Sugar Drip, respectively. In addition, the average shoot number/callus indicated 1.7, 2.9, and 3.0 in Rex, Ramada, and Sugar Drip, respectively. The survival rate of plantlets in the greenhouse, ranging from 79 to 100%, demonstrates the success of the acclimatization process and the effectiveness of the tissue culture protocol. In conclusion, this work will facilitate augmenting the efficiency of sweet sorghum transformation, improving its economical and nutritional values.
First Page
618
Last Page
630
Recommended Citation
APA Citation
Abushal, L.
Assem, S.
&
Fouad, W.
(2025). Development of an efficient indirect somatic embryogenesis and shoot regeneration system for sweet sorghum cultivars using immature inflorescence. In Vitro Cellular and Developmental Biology Plant, 61(3), 618–630.
https://doi.org/10.1007/s11627-024-10496-z
MLA Citation
Abushal, Logayn T., et al.
"Development of an efficient indirect somatic embryogenesis and shoot regeneration system for sweet sorghum cultivars using immature inflorescence." In Vitro Cellular and Developmental Biology Plant, vol. 61, no. 3, 2025, pp. 618–630.
https://doi.org/10.1007/s11627-024-10496-z