Funding Number
R22-3870733873
Funding Sponsor
Royal Society of Chemistry
Fifth Author's Department
Institute of Global Health & Human Ecology
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https://doi.org/10.1039/d3ra08908h
Document Type
Research Article
Publication Title
RSC Advances
Publication Date
3-14-2024
doi
10.1039/d3ra08908h
Abstract
Combining sonochemistry with phytochemistry is a modern trend in the biosynthesis of metallic nanoparticles (NPs), which contributes to the sustainability of chemical processes and minimizes hazardous effects. Herein, titanium dioxide (TiO2) NPs were bioengineered using a novel and facile ultrasound-assisted approach utilizing the greenly extracted essential oil of Ocimum basilicum. FTIR and UV-Vis spectrophotometry were used to confirm the formation of TiO2 NPs. The X-ray diffraction (XRD) analysis showed the crystalline nature of TiO2 NPs. TEM analysis revealed the spherical morphology of the NPs with sizes ranging from 5.55 to 13.89 nm. Energy-dispersive X-ray (EDX) confirmed the purity of the greenly synthesized NPs. TiO2 NPs demonstrated outstanding antitumor activity against breast (MCF-7) and lung (A-549) cancer cells with estimated IC50 values of 1.73 and 4.79 μg mL−1. The TiO2 NPs were cytocompatible to normal cells (MCF-10A) with a selectivity index (SI) of 8.77 for breast and 3.17 for lung cancer. Biological assays revealed a promising potential for TiO2 NPs to induce apoptosis and arrest cells at the sub-G1 phase of the cell cycle phase in both cancer cell lines. Molecular investigations showed the ability of TiO2 NPs to increase apoptotic genes' expression (Bak and Bax) and their profound ability to elevate the expression of apoptotic proteins (caspases 3 and 7). Molecular docking demonstrated strong binding interactions for TiO2 NPs with caspase 3 and EGFR-TK targets. In conclusion, the greenly synthesized TiO2 NPs exhibited potent antitumor activity and mitochondrion-based cell death against breast and lung cancer cell lines while maintaining cytocompatibility against normal cells.
First Page
8583
Last Page
8601
Recommended Citation
APA Citation
Sedky, N.
Mahdy, N.
Abdel-Kader, N.
Abdelhady, M.
...
(2024). Facile sonochemically-assisted bioengineering of titanium dioxide nanoparticles and deciphering their potential in treating breast and lung cancers: biological, molecular, and computational-based investigations. RSC Advances, 14(12), 8583–8601.
10.1039/d3ra08908h
https://fount.aucegypt.edu/faculty_journal_articles/6332
MLA Citation
Sedky, Nada K., et al.
"Facile sonochemically-assisted bioengineering of titanium dioxide nanoparticles and deciphering their potential in treating breast and lung cancers: biological, molecular, and computational-based investigations." RSC Advances, vol. 14,no. 12, 2024, pp. 8583–8601.
https://fount.aucegypt.edu/faculty_journal_articles/6332