Bioprospecting the microbiome of Red Sea Atlantis II brine pool for peptidases and biosynthetic genes with promising antibacterial activity
Author's Department
Biology Department
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https://microbialcellfactories.biomedcentral.com/counter/pdf/10.1186/s12934-022-01835-z
Document Type
Research Article
Publication Title
Microbial Cell Factories
Publication Date
Summer 6-2-2022
doi
https://doi.org/10.1186/s12934-022-01835-z
Abstract
Background: The search for novel antimicrobial agents is crucial as antibiotic-resistant pathogens continue to emerge, rendering the available antibiotics no longer efective. Likewise, new anti-cancer drugs are needed to combat the emergence of multi-drug resistant tumors. Marine environments are wealthy sources for natural products. Additionally, extreme marine environments are interesting niches to search for bioactive natural compounds. In the current study, a fosmid library of metagenomic DNA isolated from Atlantis II Deep Lower Convective Layer (ATII LCL), was functionally screened for antibacterial activity as well as anticancer efects. Results: Two clones exhibited antibacterial efects against the marine Bacillus Cc6 strain, namely clones 102-5A and 88-1G and they were further tested against eleven other challenging strains, including six safe relatives of ESKAPE pathogens (Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter spp.), a safe relative to Mycobacterium tuberculosis and four resistant clinical isolates. Clone 88-1G resulted in clear zones of inhibition against eight bacterial strains, while clone 102-5A resulted in zones of inhibition against fve bacterial strains. The whole cell lysates of clone 88-1G showed 15% inhibition of Mtb ClpP protease -Mycobacterium tuberculosis drug target-, while whole cell lysates of clone 102-5A showed 19% inhibition of Mtb ClpP protease. Whole cell lysates from the selected clones exhibited anticancer efects against MCF-7 breast cancer cells (cell viability at 50% v/v was 46.2%±9.9 for 88-1G clone and 38%±7 for 102-5A clone), U2OS osteosarcoma cells (cell viability at 50% v/v was 64.6%±12.3 for 88-1G clone and 28.3%±1.7 for 102-5A clone) and 1BR hTERT human fbroblast cells (cell viability at 50% v/v was 74.4%±5.6 for 88-1G clone and 57.6%±8.9 for 102-5A clone). Sequencing of 102-5A and 88-1G clones, and further annotation detected putative proteases and putative biosynthetic genes in clones 102-5A and 88-1G, respectively. Conclusions: The ATII LCL metagenome hosts putative peptidases and biosynthetic genes that confer antibiotic and anti-cancer efects. The tested clones exhibited promising antibacterial activities against safe relative strains to ESKAPE pathogens and Mycobacterium tuberculosis. Thus, searching the microbial dark matter of extreme environments is a promising approach to identify new molecules with pharmaceutical potential use
First Page
1
Last Page
13
Recommended Citation
Ziko, L., AbdelRaheem, O., Nabil, M. et al. Bioprospecting the microbiome of Red Sea Atlantis II brine pool for peptidases and biosynthetic genes with promising antibacterial activity. Microb Cell Fact 21, 109 (2022). https://doi.org/10.1186/s12934-022-01835-z