Production of bioactive compounds from the sulfated polysaccharides extracts of Ulva lactuca: Post-extraction enzymatic hydrolysis followed by ion-exchange chromatographic fractionation

Author's Department

Chemistry Department

Second Author's Department

Chemistry Department

Third Author's Department

Chemistry Department

Fourth Author's Department

Biology Department

Fifth Author's Department

Biology Department

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https://pubmed.ncbi.nlm.nih.gov/31195764/

All Authors

Nihal Abou El Azm; Daisy Fleita; Dalia Rifaat; Eric Zadok Mpingirika; Asma Amleh; Mayyada M. H. El-Sayed

Document Type

Research Article

Publication Title

Molecules

Publication Date

1-1-2019

doi

10.3390/molecules24112132

Abstract

This paper describes a novel combined post‐extraction process for obtaining bioactive compounds from the aqueous high molecular weight sulfated polysaccharides (SPs) extracts of the green algae, Ulva lactuca. After extracting the SPs, they were enzymatically hydrolyzed then the hydrolysate (V45) was fractionated into eight different molecular weight fractions (F1–F8) using ion exchange chromatography. Crude SPs together with V45 and (F1–F8) were examined for their carbohydrate, protein, and sulfate contents. In addition, their degree of polymerization (DP) was estimated and they were characterized by Fourier Transform Infrared Spectroscopy (FTIR). Fractions S1, F4, F5, and F8 showed promising antioxidant and antitumor activities in vitro. In particular, the remarkable antitumor activity of F5 on three types of cancer cell lines could be attributed to its comparable contents of protein, carbohydrate, and sulfate, in addition to its comparable contents of rhamnose and glucuronic acid, and the same for glucose and arabinose. F5 also possessed the highest Hill coefficient among the four promising fractions indicating a higher degree of cooperativity in ligand binding. Other influencing factors including DP, composition, and type of characteristic functional groups were also discussed. The implications of this work could potentially benefit the industries of food supplements and pharmaceuticals.

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