Influence of "glow discharge plasma" as an external stimulus on the self-assembly, morphology and binding affinity of gold nanoparticle-streptavidin conjugates
Author's Department
Chemistry Department
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https://doi.org/10.3390/ijms13066534
Document Type
Research Article
Publication Title
International Journal of Molecular Sciences
Publication Date
6-1-2012
doi
10.3390/ijms13066534
Abstract
In this study, we investigate the influence of glow discharge plasma (GDP) on the self-assembly, morphology and binding affinity of streptavidin coated gold nanoparticles (Au-NP-SV) and biotinylated antibody (bAb) adsorbed on a highly oriented pyrolytic graphite (HOPG) substrate. Atomic force microscope (AFM) was used to image the pre- and post-GDP treated samples. The analysis of the AFM images showed a considerable change in the aggregation and morphology of Au-NP-conjugates after treatment with GDP. To our knowledge, this is the first report on using GDP to enhance and speed-up the aggregation (sintering) of adsorbed NP biomolecular conjugates. These results show a promising route that could be generalized for other NPs and their conjugates. It can also be considered as an alternative and cheap aggregation method for controlling the binding affinity of biomolecular species on different surfaces with interesting applications. © 2012 by the authors; licensee MDPI, Basel, Switzerland.
First Page
6534
Last Page
6547
Recommended Citation
APA Citation
Mamdouh, W.
Li, Y.
Shawky, S.
Azzazy, H.
&
Liu, C.
(2012). Influence of "glow discharge plasma" as an external stimulus on the self-assembly, morphology and binding affinity of gold nanoparticle-streptavidin conjugates. International Journal of Molecular Sciences, 13(6), 6534–6547.
10.3390/ijms13066534
https://fount.aucegypt.edu/faculty_journal_articles/2137
MLA Citation
Mamdouh, Wael, et al.
"Influence of "glow discharge plasma" as an external stimulus on the self-assembly, morphology and binding affinity of gold nanoparticle-streptavidin conjugates." International Journal of Molecular Sciences, vol. 13,no. 6, 2012, pp. 6534–6547.
https://fount.aucegypt.edu/faculty_journal_articles/2137