Effect of ultrasonic treatment on the morphology of casein particles

Funding Number

08JDG039

Funding Sponsor

Natural Science Foundation of Jiangsu Province

Author's Department

Chemistry Department

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https://doi.org/10.1016/j.ultsonch.2013.08.017

Document Type

Research Article

Publication Title

Ultrasonics Sonochemistry

Publication Date

1-1-2014

doi

10.1016/j.ultsonch.2013.08.017

Abstract

In this study, the effect of ultrasonic treatment duration on the morphology of self-assembled casein particles was investigated by atomic force microscopy (AFM), low vacuum scanning electron microscopy (SEM) and transmission electron microscopy (TEM). In the case of AFM images, the particle analysis which was carried out by the SPIP program showed that the self-assembled casein particles after being ultrasonically treated for 2 min got smaller in size compared to the casein particles that have not been exposed to any ultrasonic treatment. Surprisingly, however, increasing the ultrasonic time exposure of the particles resulted in an opposite effect where larger particles or aggregates seemed to be present. We show that by comparing the results obtained by AFM, SEM and TEM, the information extracted from the AFM images and analyzed by SPIP program give more detailed insights into particle sizes and morphology at the molecular level compared to SEM and TEM images, respectively. © 2013 Elsevier B.V. All rights reserved.

First Page

513

Last Page

519

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