Direct detection of unamplified hepatoma upregulated protein RNA in urine using gold nanoparticles for bladder cancer diagnosis
Funding Number
21/2
Author's Department
Chemistry Department
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https://doi.org/10.1016/j.clinbiochem.2013.10.022
Document Type
Research Article
Publication Title
Clinical Biochemistry
Publication Date
1-1-2014
doi
10.1016/j.clinbiochem.2013.10.022
Abstract
Objective: To develop a gold nanoparticle (AuNP) assay for direct detection of unamplified HURP RNA in urine. Design and methods: HURP RNA was extracted from urine samples (50 bladder carcinoma patients, 25 benign bladder lesions, and 25 controls) and further purified using magnetic nanoparticles (MNPs), functionalized with HURP RNA-specific oligonucleotides, and then detected by RT-PCR or gold nanoparticles. Results: The developed HURP RNA AuNP assay has a sensitivity and a specificity of 88.5% and 94%, respectively, and a detection limit of 2.4. nmol/L. The concordance between the HURP AuNP assay with RT-PCR after RNA purification using functionalized MNPs was 97%. Conclusions: The developed colorimetric HURP RNA AuNP assay is sensitive, simple, and can aid noninvasive diagnosis of bladder cancer. © 2013 The Canadian Society of Clinical Chemists.
First Page
104
Last Page
110
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APA Citation
Eissa, S.
Shawky, S.
Matboli, M.
Mohamed, S.
&
Azzazy, H. M.
(2014). Direct detection of unamplified hepatoma upregulated protein RNA in urine using gold nanoparticles for bladder cancer diagnosis. Clinical Biochemistry, 47(1-2), 104–110.
10.1016/j.clinbiochem.2013.10.022
https://fount.aucegypt.edu/faculty_journal_articles/1873
MLA Citation
Eissa, Sanaa, et al.
"Direct detection of unamplified hepatoma upregulated protein RNA in urine using gold nanoparticles for bladder cancer diagnosis." Clinical Biochemistry, vol. 47,no. 1-2, 2014, pp. 104–110.
https://fount.aucegypt.edu/faculty_journal_articles/1873