Abstract
The objective of the current study was the assessment of heterogeneity amongst metabolites of 5 cereal and leguminous grains of different species, and cultivars i.e., Triticum aestivum L., Hordeum vulgare L., Vicia faba L. & Cicer arietinum L. at different maturity stages. Gas chromatography mass-spectrometry (GC-MS) post silylation technique was employed for nutrients profiling. A total of 70 peaks belonging to sugars, fatty acids/esters/steroids and organic acids were identified including sucrose, melibiose, glucose and fructose as major sugars, butyl caprylate, hydroxybutanoic acid and malic acid to contribute for the discrimination between grain species and different maturity stages. Ultra-performance liquid chromatography tandem mass- spectrometry (UPLC-MS/MS) was further applied for secondary metabolites’ profiling. A total of 147 peaks were annotated from various classes viz. sugars, oxylipids, flavonoids, amino acids/peptides, terpenoids, and alkaloids. Datasets from both techniques were coupled to chemometric analysis for better processing and identification of markers. Molecular networking was applied in UPLC-MS dataset for better visualization and cluster segregation. DPPH and FRAP assays were attempted to correlate antioxidant results to secondary metabolites’ variation upon maturation.
School
School of Sciences and Engineering
Department
Chemistry Department
Degree Name
MS in Chemistry
Graduation Date
Winter 1-15-2023
Submission Date
10-10-2022
First Advisor
Mohamed Ali Farag
Committee Member 1
Hatem Tallima
Committee Member 2
Nesrine Hegazi
Committee Member 3
Mohamed Salama
Extent
104 p.
Document Type
Master's Thesis
Institutional Review Board (IRB) Approval
Not necessary for this item
Recommended Citation
APA Citation
Saied, D. B.
(2023).Comparative Metabolite Profiling and Fingerprinting of Mature and Immature Grains of Different Species using a Multiplex Approach of LC-MS and GC-MS Coupled to Chemometrics [Master's Thesis, the American University in Cairo]. AUC Knowledge Fountain.
https://fount.aucegypt.edu/etds/1978
MLA Citation
Saied, Doaa Bahaa Abdelhalim. Comparative Metabolite Profiling and Fingerprinting of Mature and Immature Grains of Different Species using a Multiplex Approach of LC-MS and GC-MS Coupled to Chemometrics. 2023. American University in Cairo, Master's Thesis. AUC Knowledge Fountain.
https://fount.aucegypt.edu/etds/1978