CRISPR Del/Rei: a simple, flexible, and efficient pipeline for scarless genome editing

Kyra L. Feuer, The American University in Cairo (AUC)
Marah H. Wahbeh
Christian Yovo
Eman Rabie, The American University in Cairo (AUC)


Scarless genome editing of induced pluripotent stem cells (iPSCs) is crucial for the precise modeling of genetic disease. Here we present CRISPR Del/Rei, a two-step deletion-reinsertion strategy with high editing efficiency and simple PCR-based screening that generates isogenic clones in ~ 2 months. We apply our strategy to edit iPSCs at 3 loci with only rare off target editing.