Green Synthesis of Platinum and Palladium Nanoparticles Using L. Seed Alkaloids: Biological and Computational Studies

Author's Department

Chemistry Department

Find in your Library

All Authors

Sherif Ashraf Fahmy; Iten M. Fawzy; Basma M. Saleh; Marwa Y. Issa; Udo Bakowsky; Hassan Mohamed El-Said Azzazy

Document Type

Research Article

Publication Title

Nanomaterials (Basel, Switzerland)

Publication Date





This study reports a facile and eco-friendly method for the green synthesis of platinum and palladium nanoparticles (Pt NPs and Pd NPs) using seed alkaloid fraction. The ζ-potential of the synthesized Pt NPs, Pd NPs and Pt-Pd NPs were -11.2 ± 0.5, -9.7 ±1.2, and -12.7 ± 2.1 mV; respectively. Transmission electron microscopy (TEM) revealed the formation of spherical-shaped nanoparticles with smooth margins. The mean diameters of the synthesized Pt NPs, Pd NPs, and Pt-Pd NPs were determined using TEM analysis and were found to be 20.3 ± 1.9, 22.5 ± 5.7, and 33.5 ± 5.4 nm, respectively. The nanoparticles' bioreduction was confirmed by ultraviolet-visible (UV-vis) spectroscopy, X-ray diffraction (XRD) and Fourier transform infrared (FTIR) spectroscopy, and their organic contents were determined by thermal gravimetric analysis (TGA). The Pt-Pd NPs mixture showed more pronounced antioxidant activity of 843.0 ± 60 μM Trolox equivalent (TE)/mg NPs compared to the individual Pt NPs (277.3 ± 13.5 μM TE/mg NPs) and Pd NPs (167.6 ± 4.8 μM TE/mg NPs). Furthermore, the Pt-Pd NPs exhibited significant cytotoxic activities against lung cancer (A549) and breast adenocarcinoma (MCF-7) cells, IC of 8.8 and 3.6 µg/mL, respectively; as compared to Pt NPs (IC of 10.9 and 6.7 µg/mL, respectively) and Pd NPs (IC of 31 and 10.8 µg/mL, respectively and compared to carboplatin (IC of 23 and 9.5 µg/mL, respectively). Moreover, molecular docking studies were conducted to explore the possible anticancer and antioxidant mechanisms of the biogenic nanoparticles. Pt NPs, Pd NPs, and their mixture showed inhibitory activity against cysteine proteinase, which supports their high antitumor activity, but moderate antioxidant activity. In conclusion, Pd-Pt NPs mixture prepared using harmala seed alkaloid fraction showed potential as effective antineoplastic agents.

First Page


Last Page


This document is currently not available here.