Diadema savignyi spine extract in an experimental setup using L929 cell line in vitro. The cell metabolic activity of L929 cells is tested through an MTT assay. The sea urchin spine extract is applied to the cells in two concentrations: 100 μg/ml (136% viability) and 200 μg/ml (95% viability). The bioactive components of the sea urchin spine are identified via GC-MS, and the antioxidant and anti-inflammatory activities are evaluated using catalase assay (CAT), glutathione (GSH), and nitric oxide (NO) tests. Results show that the GC-MS identified bioactive components including the anti-inflammatory and anti-irritant bisabolol oxide and the pro-inflammatory oleic acid. Additionally, GSH and NO tests indicate significant antioxidant actions of the sea urchin spine in both the 100 μg/ml and 200 μg/ml with roughly 0.098 and 0.103 mmol/L in GSH test, and 20.46 and 20.6 mmol/L in NO test, respectively. However, the CAT results were inconsistent with that of the GSH and NO, therefore a repetition of this test is recommended. Overall, the study concludes that the significant anti-inflammatory and antioxidant actions of the sea urchin spine qualify it as a potential candidate in wound healing ointments.
Professor Ahmed Moustafa
El Gamal, Dina Magdy
"Anti-inflammatory and Antioxidant Effects of Sea Urchin Spine Extract,"
The Undergraduate Research Journal: Vol. 9, Article 3.
Available at: https://fount.aucegypt.edu/urje/vol9/iss1/3
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