Title

CRISPR Del/Rei: a simple, flexible, and efficient pipeline for scarless genome editing

Author's Department

Biotechnology Program

Find in your Library

https://www.nature.com/articles/s41598-022-16004-w.pdf

All Authors

Kyra L. Feuer, Marah H. Wahbeh, Christian Yovo, Eman Rabie, Anh-Thu N. Lam, Sara Abdollahi, Lindsay J. Young, Bailey Rike, Akul Umamageswaran, Dimitrios Avramopoulos

Document Type

Research Article

Publication Title

Scientific Reports

Publication Date

Summer 7-13-2022

doi

https://doi.org/10.1038/s41598-022-16004-w

Abstract

Scarless genome editing of induced pluripotent stem cells (iPSCs) is crucial for the precise modeling of genetic disease. Here we present CRISPR Del/Rei, a two-step deletion-reinsertion strategy with high editing efficiency and simple PCR-based screening that generates isogenic clones in ~ 2 months. We apply our strategy to edit iPSCs at 3 loci with only rare off target editing.

First Page

1

Last Page

4

This document is currently not available here.

Find this item online

Share

COinS