CRISPR Del/Rei: a simple, flexible, and efficient pipeline for scarless genome editing

Authors

Kyra L. Feuer, The American University in Cairo (AUC)
Marah H. Wahbeh
Christian Yovo
Eman Rabie, The American University in Cairo (AUC)Follow

Author's Department

Biotechnology Program

Find in your Library

https://doi.org/10.1038/s41598-022-16004-w

All Authors

Kyra L. Feuer, Marah H. Wahbeh, Christian Yovo, Eman Rabie, Anh-Thu N. Lam, Sara Abdollahi, Lindsay J. Young, Bailey Rike, Akul Umamageswaran, Dimitrios Avramopoulos

Document Type

Research Article

Publication Title

Scientific Reports

Publication Date

Summer 7-13-2022

doi

10.1038/s41598-022-16004-w

Abstract

Scarless genome editing of induced pluripotent stem cells (iPSCs) is crucial for the precise modeling of genetic disease. Here we present CRISPR Del/Rei, a two-step deletion-reinsertion strategy with high editing efficiency and simple PCR-based screening that generates isogenic clones in ~ 2 months. We apply our strategy to edit iPSCs at 3 loci with only rare off target editing.

First Page

1

Last Page

4

Recommended Citation

Feuer, K.L., Wahbeh, M.H., Yovo, C. et al. CRISPR Del/Rei: a simple, flexible, and efficient pipeline for scarless genome editing. Sci Rep 12, 11928 (2022). https://doi.org/10.1038/s41598-022-16004-w