CRISPR Del/Rei: a simple, flexible, and efficient pipeline for scarless genome editing
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Scarless genome editing of induced pluripotent stem cells (iPSCs) is crucial for the precise modeling of genetic disease. Here we present CRISPR Del/Rei, a two-step deletion-reinsertion strategy with high editing efficiency and simple PCR-based screening that generates isogenic clones in ~ 2 months. We apply our strategy to edit iPSCs at 3 loci with only rare off target editing.
Feuer, K.L., Wahbeh, M.H., Yovo, C. et al. CRISPR Del/Rei: a simple, flexible, and efficient pipeline for scarless genome editing. Sci Rep 12, 11928 (2022). https://doi.org/10.1038/s41598-022-16004-w