Title

Direct detection of unamplified hepatoma upregulated protein RNA in urine using gold nanoparticles for bladder cancer diagnosis

Funding Number

21/2

Author's Department

Chemistry Department

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https://doi.org/10.1016/j.clinbiochem.2013.10.022

Document Type

Research Article

Publication Title

Clinical Biochemistry

Publication Date

1-1-2014

doi

10.1016/j.clinbiochem.2013.10.022

Abstract

Objective: To develop a gold nanoparticle (AuNP) assay for direct detection of unamplified HURP RNA in urine. Design and methods: HURP RNA was extracted from urine samples (50 bladder carcinoma patients, 25 benign bladder lesions, and 25 controls) and further purified using magnetic nanoparticles (MNPs), functionalized with HURP RNA-specific oligonucleotides, and then detected by RT-PCR or gold nanoparticles. Results: The developed HURP RNA AuNP assay has a sensitivity and a specificity of 88.5% and 94%, respectively, and a detection limit of 2.4. nmol/L. The concordance between the HURP AuNP assay with RT-PCR after RNA purification using functionalized MNPs was 97%. Conclusions: The developed colorimetric HURP RNA AuNP assay is sensitive, simple, and can aid noninvasive diagnosis of bladder cancer. © 2013 The Canadian Society of Clinical Chemists.

First Page

104

Last Page

110

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