Title

Fabrication of layer-by-layer ECM/polysaccharides/electrospun composite nanofibrous scaffolds and hydrogels for in-vitro skin cell regeneration

Abstract

Skin burn wounds is a crucial issue that could reduce life quality. The novel tri-layered asymmetric porous scaffold has been innovated to mimic the natural skin layers. The scaffold constitutes of upper electrospun Cs-PVA layer and lower xerogel layer that made of effective skin ECM components. Both layers were stuck together by fibrin glue. The developed scaffold resulted in promising swelling capability suitable for absorbing wound exudates, followed by constant degradable weight over time which is appropriate for burn wounds environment. SEM images revealed the presence of significant pore distribution among the electrospun and xerogel layers confirmed by BET analysis. The electrospun nanofibrous layer was examined for its antibacterial property and showed expressive complete bacterial inhibition against gram-negative and gram-positive bacterial strains. Mouse embryonic fibroblast cytotoxicity and migration rate were investigated against the created asymmetrical composite. The outcome results of tissue culture experiments demonstrated significant cell proliferation and migration in presence of the constructed scaffold (P<0.0001). Complete wound closure was observed in-vitro in presence of the three scaffold asymmetrical layers against mouse embryonic fibroblast. Results of this study have been proved the superior biological characteristics of the innovated asymmetrical composite, that could further replace the burned skin layers with potential for clinical applications.

Department

Biotechnology Program

Degree Name

MS in Biotechnology

Graduation Date

2-1-2019

Submission Date

January 2019

First Advisor

Sayed, Wael Mamdouh

Committee Member 1

Al-Ghobashy, Medhat

Committee Member 2

Tallima, Hatem

Extent

169 p.

Document Type

Master's Thesis

Rights

The author retains all rights with regard to copyright. The author certifies that written permission from the owner(s) of third-party copyrighted matter included in the thesis, dissertation, paper, or record of study has been obtained. The author further certifies that IRB approval has been obtained for this thesis, or that IRB approval is not necessary for this thesis. Insofar as this thesis, dissertation, paper, or record of study is an educational record as defined in the Family Educational Rights and Privacy Act (FERPA) (20 USC 1232g), the author has granted consent to disclosure of it to anyone who requests a copy.

Institutional Review Board (IRB) Approval

Not necessary for this item

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