Abstract

D1S80 (1p35-p36), variable Number of tandem repeat (VNTR,) is a minisatellite molecular genetic marker. It is known by its high polymorphism that makes it a powerful marker in the forensic applications, paternity testing, and evolutionary studies. Hypervariable D1S80 locus has been applied to study the genetic structure of the Nubian population in Southern Egypt. PCR has been applied for repeats amplification and analyzed on 2% agarose gel. 75 genotypes and 36 alleles with their frequencies were detected for 105 unrelated individuals. Allele 27 has the highest frequency 0.119% followed by allele 26 with frequency 0.09%. Heterozygosity was calculated as 0.69%. P values of Chi2 and exact tests indicate departure from Hardy-Weinberg equilibrium which points to the inbreeding within the population. Non-metric dimensional scaling and hierarchical cluster dedrograms were applied to determine the phylogenetic relationship between the Nubian population and other worldwide populations. Both plots indicated the proximity of the Nubian population to Modern Malay population as a result of dominance of 27 alleles in both populations.

Department

Biotechnology Program

Degree Name

MS in Biotechnology

Graduation Date

2-1-2016

Submission Date

September 2016

First Advisor

El Dorry, Hamza

Committee Member 1

Mahmoud, Hamada

Committee Member 2

Kakarougkas, Andreas

Extent

67 p.

Document Type

Master's Thesis

Rights

The author retains all rights with regard to copyright. The author certifies that written permission from the owner(s) of third-party copyrighted matter included in the thesis, dissertation, paper, or record of study has been obtained. The author further certifies that IRB approval has been obtained for this thesis, or that IRB approval is not necessary for this thesis. Insofar as this thesis, dissertation, paper, or record of study is an educational record as defined in the Family Educational Rights and Privacy Act (FERPA) (20 USC 1232g), the author has granted consent to disclosure of it to anyone who requests a copy.

Institutional Review Board (IRB) Approval

Approval has been obtained for this item

Comments

Mo Ibrahim Foundation

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